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l6 rat myoblast cell line  (ATCC)


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    ATCC l6 rat myoblast cell line
    L6 Rat Myoblast Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 398 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/l6+rat+myoblasts/L6%3B+Myoblast%3B+Rat/pm41779271-45-1-10
    Average 95 stars, based on 398 article reviews
    l6 rat myoblast cell line - by Bioz Stars, 2026-10
    95/100 stars

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    Cell Culture:

    Article Title: Protective Effects of Quinic Acid Against Disuse-Induced Skeletal Muscle Atrophy via Regulation of Inflammation and Oxidative Stress
    Article Snippet: Serum samples were prepared by centrifugation of collected blood at 1300× g for 15 min, and the concentration of IL-15 was determined using a commercial ELISA kit (Merck Millipore, Burlington, MA, USA, manufactured in Germany) in accordance with the manufacturer’s protocol. .. L6 rat myoblasts (ATCC, Manassas, VA, USA) were cultured as previously described [ ]. .. To induce differentiation, cells at approximately 70–80% confluence were switched from growth medium to differentiation medium composed of Dulbecco’s Modified Eagle’s Medium (HyClone, Logan, UT, USA) supplemented with 1% horse serum (Gibco, Gaithersburg, MD, USA) and 100 U/mL penicillin with 100 μg/mL streptomycin.

    Article Title: Mesenchymal stromal cell spheroids in sulfated alginate enhance muscle regeneration
    Article Snippet: .. L6 rat myoblasts (ATCC, Manassas, VA) were cultured in DMEM (Invitrogen) supplemented with 10% FBS, 1% P/S. ..



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    Concentration-dependent cytotoxicity of carvacrol in L6 rat myoblasts. Cells were treated with increasing concentrations of carvacrol (0.75–100 µg/mL) for 24 h in the absence of TNF-α, and viability was assessed using the MTT assay. Note the mild but consistent reduction in viability at 6.25 µg/mL relative to control, and the marked concentration-dependent decline above 12.5 µg/mL approaching the IC 50 (∼60 µg/mL). Data are presented as mean ± SEM (n = 3). *** p < 0.001 vs. control; ** p < 0.01 vs. control; * p < 0.05 vs. control (one-way ANOVA, Tukey’s HSD).

    Journal: International Journal of Molecular Sciences

    Article Title: Ion-Dependent ATPase Activity and Metabolic Gene Expression in TNF-α-Challenged Skeletal Muscle Cells: Mechanistic Characterisation of Carvacrol’s Bioenergetic Effects

    doi: 10.3390/ijms27104511

    Figure Lengend Snippet: Concentration-dependent cytotoxicity of carvacrol in L6 rat myoblasts. Cells were treated with increasing concentrations of carvacrol (0.75–100 µg/mL) for 24 h in the absence of TNF-α, and viability was assessed using the MTT assay. Note the mild but consistent reduction in viability at 6.25 µg/mL relative to control, and the marked concentration-dependent decline above 12.5 µg/mL approaching the IC 50 (∼60 µg/mL). Data are presented as mean ± SEM (n = 3). *** p < 0.001 vs. control; ** p < 0.01 vs. control; * p < 0.05 vs. control (one-way ANOVA, Tukey’s HSD).

    Article Snippet: L6 rat skeletal myoblasts (ATCC CRL-1458; RRID:CVCL_0385) were cultured in RPMI-1640 medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% heat-inactivated foetal bovine serum (FBS) and 1% penicillin–streptomycin.

    Techniques: Concentration Assay, MTT Assay, Control

    Cytoprotective effect of post-TNF-α carvacrol treatment on L6 myoblast viability. Cells were pre-exposed to TNF-α (10 ng/mL, 1 h) to induce inflammatory stress, followed by treatment with carvacrol at the indicated concentrations for 24 h. Cell viability was assessed using the MTT assay. Data are presented as mean ± SEM (n = 3). *** p < 0.001 vs. untreated control (one-way ANOVA, Tukey’s HSD).

    Journal: International Journal of Molecular Sciences

    Article Title: Ion-Dependent ATPase Activity and Metabolic Gene Expression in TNF-α-Challenged Skeletal Muscle Cells: Mechanistic Characterisation of Carvacrol’s Bioenergetic Effects

    doi: 10.3390/ijms27104511

    Figure Lengend Snippet: Cytoprotective effect of post-TNF-α carvacrol treatment on L6 myoblast viability. Cells were pre-exposed to TNF-α (10 ng/mL, 1 h) to induce inflammatory stress, followed by treatment with carvacrol at the indicated concentrations for 24 h. Cell viability was assessed using the MTT assay. Data are presented as mean ± SEM (n = 3). *** p < 0.001 vs. untreated control (one-way ANOVA, Tukey’s HSD).

    Article Snippet: L6 rat skeletal myoblasts (ATCC CRL-1458; RRID:CVCL_0385) were cultured in RPMI-1640 medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% heat-inactivated foetal bovine serum (FBS) and 1% penicillin–streptomycin.

    Techniques: MTT Assay, Control

    Ion-dependent ATPase activities following TNF-α exposure and carvacrol treatment. L6 myoblasts were exposed to TNF-α (10 ng/mL, 1 h) followed by carvacrol (6.25 µg/mL, 24 h). Activities of Na + /K + -dependent, Ca 2+ -dependent, and Mg 2+ -dependent ATPases were assessed as ion-dependent ATP hydrolysis capacity by inorganic phosphate release. Data are mean ± SEM (n = 3). *** p < 0.001; ** p < 0.01; * p < 0.05 (one-way ANOVA, Tukey’s HSD).

    Journal: International Journal of Molecular Sciences

    Article Title: Ion-Dependent ATPase Activity and Metabolic Gene Expression in TNF-α-Challenged Skeletal Muscle Cells: Mechanistic Characterisation of Carvacrol’s Bioenergetic Effects

    doi: 10.3390/ijms27104511

    Figure Lengend Snippet: Ion-dependent ATPase activities following TNF-α exposure and carvacrol treatment. L6 myoblasts were exposed to TNF-α (10 ng/mL, 1 h) followed by carvacrol (6.25 µg/mL, 24 h). Activities of Na + /K + -dependent, Ca 2+ -dependent, and Mg 2+ -dependent ATPases were assessed as ion-dependent ATP hydrolysis capacity by inorganic phosphate release. Data are mean ± SEM (n = 3). *** p < 0.001; ** p < 0.01; * p < 0.05 (one-way ANOVA, Tukey’s HSD).

    Article Snippet: L6 rat skeletal myoblasts (ATCC CRL-1458; RRID:CVCL_0385) were cultured in RPMI-1640 medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% heat-inactivated foetal bovine serum (FBS) and 1% penicillin–streptomycin.

    Techniques:

    LDH release following TNF-α exposure and carvacrol treatment. L6 myoblasts were exposed to TNF-α (10 ng/mL, 1 h) followed by carvacrol (6.25 µg/mL, 24 h). LDH activity in culture supernatants was quantified as an index of membrane damage. Data are mean ± SEM (n = 3). *** p < 0.001 (one-way ANOVA, Tukey’s HSD).

    Journal: International Journal of Molecular Sciences

    Article Title: Ion-Dependent ATPase Activity and Metabolic Gene Expression in TNF-α-Challenged Skeletal Muscle Cells: Mechanistic Characterisation of Carvacrol’s Bioenergetic Effects

    doi: 10.3390/ijms27104511

    Figure Lengend Snippet: LDH release following TNF-α exposure and carvacrol treatment. L6 myoblasts were exposed to TNF-α (10 ng/mL, 1 h) followed by carvacrol (6.25 µg/mL, 24 h). LDH activity in culture supernatants was quantified as an index of membrane damage. Data are mean ± SEM (n = 3). *** p < 0.001 (one-way ANOVA, Tukey’s HSD).

    Article Snippet: L6 rat skeletal myoblasts (ATCC CRL-1458; RRID:CVCL_0385) were cultured in RPMI-1640 medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% heat-inactivated foetal bovine serum (FBS) and 1% penicillin–streptomycin.

    Techniques: Activity Assay, Membrane

    Catalase and superoxide dismutase (SOD) activities following TNF-α exposure and carvacrol treatment. L6 myoblasts were exposed to TNF-α (10 ng/mL, 1 h) followed by carvacrol (6.25 µg/mL, 24 h). Enzyme activities were normalised to total protein content (Bradford assay). Data are mean ± SEM (n = 3). *** p < 0.001; ** p < 0.01; * p < 0.05 (one-way ANOVA, Tukey’s HSD).

    Journal: International Journal of Molecular Sciences

    Article Title: Ion-Dependent ATPase Activity and Metabolic Gene Expression in TNF-α-Challenged Skeletal Muscle Cells: Mechanistic Characterisation of Carvacrol’s Bioenergetic Effects

    doi: 10.3390/ijms27104511

    Figure Lengend Snippet: Catalase and superoxide dismutase (SOD) activities following TNF-α exposure and carvacrol treatment. L6 myoblasts were exposed to TNF-α (10 ng/mL, 1 h) followed by carvacrol (6.25 µg/mL, 24 h). Enzyme activities were normalised to total protein content (Bradford assay). Data are mean ± SEM (n = 3). *** p < 0.001; ** p < 0.01; * p < 0.05 (one-way ANOVA, Tukey’s HSD).

    Article Snippet: L6 rat skeletal myoblasts (ATCC CRL-1458; RRID:CVCL_0385) were cultured in RPMI-1640 medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% heat-inactivated foetal bovine serum (FBS) and 1% penicillin–streptomycin.

    Techniques: Bradford Assay

    Relative mRNA expression of SIRT1 and AMPK following TNF-α exposure and carvacrol treatment. L6 myoblasts were exposed to TNF-α (10 ng/mL, 1 h) followed by carvacrol (6.25 µg/mL, 24 h). mRNA expression was quantified by quantitative RT-PCR using the 2 −ΔΔCt method and normalised to GAPDH. Data are expressed as fold change relative to the TNF-α-treated group and presented as mean ± SEM (n = 3). Significance level: ** p < 0.01; * p < 0.05 (one-way ANOVA, Tukey’s HSD).

    Journal: International Journal of Molecular Sciences

    Article Title: Ion-Dependent ATPase Activity and Metabolic Gene Expression in TNF-α-Challenged Skeletal Muscle Cells: Mechanistic Characterisation of Carvacrol’s Bioenergetic Effects

    doi: 10.3390/ijms27104511

    Figure Lengend Snippet: Relative mRNA expression of SIRT1 and AMPK following TNF-α exposure and carvacrol treatment. L6 myoblasts were exposed to TNF-α (10 ng/mL, 1 h) followed by carvacrol (6.25 µg/mL, 24 h). mRNA expression was quantified by quantitative RT-PCR using the 2 −ΔΔCt method and normalised to GAPDH. Data are expressed as fold change relative to the TNF-α-treated group and presented as mean ± SEM (n = 3). Significance level: ** p < 0.01; * p < 0.05 (one-way ANOVA, Tukey’s HSD).

    Article Snippet: L6 rat skeletal myoblasts (ATCC CRL-1458; RRID:CVCL_0385) were cultured in RPMI-1640 medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% heat-inactivated foetal bovine serum (FBS) and 1% penicillin–streptomycin.

    Techniques: Expressing, Quantitative RT-PCR